Journal: STAR Protocols

Article Title: Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells

doi: 10.1016/j.xpro.2022.101974

Figure Lengend Snippet: SnapGene map of the pGEN2-DEST plasmid containing GalNAc-T7 as an example, as cloned and provided by Moremen and colleagues. The BH GalNAc-T7 version of this construct was successfully expressed, purified and used in in vitro glycosylation experiments

Article Snippet: We use the In-Fusion Cloning action in SnapGene to simplify PCR primer design ( https://www.snapgene.com/resources/in-fusion-cloning/?referrer=SnapGene ). b.

Techniques: Plasmid Preparation, Clone Assay, Construct, Purification, In Vitro, Glycoproteomics

Journal: STAR Protocols

Article Title: Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9

doi: 10.1016/j.xpro.2025.103949

Figure Lengend Snippet: Schematic of a target HDR donor plasmid for N-terminal tagging and suggested sequencing (seq) primers Left homology arm (LHA), right homology arm (RHA), AB R (antibiotic resistance cassette), se (sense), ase (anti-sense), L (glycine-serine linker). Map is visualized using the SnapGene software.

Article Snippet: The primers must contain 20–25 bp overlapping with the adjacent gene fragment or vector to be cloned using Gibson assembly. d. Perform an in silico Gibson assembly using cloning design software, such as SnapGene or Benchling.

Techniques: Plasmid Preparation, Sequencing, Software

Journal: STAR Protocols

Article Title: Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9

doi: 10.1016/j.xpro.2025.103949

Figure Lengend Snippet: Schematic of a target HDR donor plasmid for C-terminal tagging and suggested sequencing (seq) primers Left homology arm (LHA), right homology arm (RHA), AB R (antibiotic resistance cassette), se (sense), ase (anti-sense), L (glycine-serine linker). Map is visualized using the SnapGene software.

Article Snippet: The primers must contain 20–25 bp overlapping with the adjacent gene fragment or vector to be cloned using Gibson assembly. d. Perform an in silico Gibson assembly using cloning design software, such as SnapGene or Benchling.

Techniques: Plasmid Preparation, Sequencing, Software

Journal: International Journal of Molecular Sciences

Article Title: Localization and Molecular Cloning of the ASMT Gene for Melatonin Synthesis in Pigs

doi: 10.3390/ijms26020606

Figure Lengend Snippet: Sequencing results and alignment analysis of the pig ASMT gene cloning. ( A ) The alignment of the cloned pig ASMT gene sequence with the reference sequence. ( B ) The Sanger sequencing chromatogram of the cloned pig ASMT gene sequence. ( C ) Visualization of the exon information of the cloned pig ASMT gene using SnapGene software. ( D ) Comparison of human ASMT gene transcripts and the existing and newly cloned pig ASMT transcripts in NCBI.

Article Snippet: (1) Gene Query and Analysis Websites: NCBI ( https://ncbi.nlm.nih.gov/ ); Ensembl ( https://asia.ensembl.org/ ); UCSC Genome Browser ( http://genome.ucsc.edu/ ). (2) Primer Design Website: Primer 3.0 ( https://primer3.ut.ee ). (3) Vector Design and Sequence Alignment Software: SnapGene.

Techniques: Sequencing, Clone Assay, Software, Comparison